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1. Library quantity
2. Library quality

Archive

Archive 2019 - Wetlab Training

Class II. NGS - I

2. How to check the quantity and quality of RNA-seq library

Last updated 3 years ago

1. Library quantity

Qubit dsDNA kit
Agilent 2100

2. Library quality

Agilent 2100
qPCR

Using qPCR primer pairs to target the P5/P7 and spike-in sequences, only the libraries constructed successfully could be amplyfied.